Personal Information

Name: Wijerupage Sandhya Sulochana Wijesundera

Designation: Senior Lecturer Grade 1

 

Academic Record

·         B.Sc. Hons., [Colombo], 1980. University of Colombo, Sri Lanka, Second Class Upper Division. Special Degree in Chemistry with Botany as the subsidiary subject.

·         M.Sc. [Bristol], 1984. University of Bristol, England, Department of Organic Chemistry in Bio-organic Chemistry.

·         Ph.D.  [Colombo], 1997. University of Colombo, Sri Lanka in Molecular Biology & Gene Technology.

Research Interests  

  • Molecular Biology of filarial parasites
  • DNA based diagnosis of infectious diseases
  • DNA based diagnosis of genetic diseases
  • Gene manipulations in plants (Rice, Papaya)
  • Study of polymorphism in plant pathogenic fungi using molecular markers
  • Study of gene manipulations in fungi

 Publications

 W.S.S. Wijesundera (2009). 'Recombinant DNA technology: Basic Tools and Techniques'

 ISBN-978-955-52088-0-2 

 W.S.S. Wijesundera, (2001). ‘Setariosis’ in Service, M.W. ed.  “The Encyclopedia of Arthropod transmitted infections”, pages 465-468, CABI publishing.

List of Publications

Jayasinghe D. R. and Wijesundera W. S. S. (2003).  Differentiation of Setaria digitata and Setaria labiatopapillosa using molecular markers.   The Veterinary Journal, 165, 136-142.

Gunawardena Y.I.N.S, Wijesundera W.S.S, Karunanayake E.H, Chandrasekharan N. V, Jayasekara N and Siridewa K. (1999). A simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti.  Southeast Asian Journal of Tropical Medicine and Public Health30, 350-355.

 

Wijesundera W. S. S., Chandrasekharan N. V., Karunanayake E. H. (1999).  A sensitive polymerase chain reaction based assay for the detection of Setaria digitata: The causative organism of cerebrospinal nematodiasis in goats, sheep and horses.  Veterinary Parasitology81, 225-233.

 

Wijesundera W. S. S., Chandrasekharan N. V., Karunanayake E. H. and Rajapakse R. P. V. J. (1997).  Development of a rapid, nonradioactive, oligonucleotide-based assay for the detection of Setaria digitata.  Experimental Parasitology86: 161-162.

 

Wijesundera W. S. S., Chandrasekharan N. V., Karunanayake E. H and Dharmasena S. P. (1996). Development of a diagnostic DNA probe to detect Setaria digitata: The causative parasite of cerebrospinal nematodiasis in goats, sheep and horses.  British Veterinary Journal152: 561-571.

Bennet, A.J., Sinnot, M.L. and Wijesundera W.S.S., [1985].  18O and Secondary 2 - arabinofuranosides. aH Kinetic isotope effects confirms the existence of two pathways for acid catalysed hydrolysis of  Journal of Chemical Society, Perkin Trans. 11, 1233-1236.

 Sinnot, M.L. and Wijesundera W.S.S., [1985].  -L- arabinofuranosyl pyridinium salts and hydrolysis of theaPreparation and conformation of  4- bromoisoquinolinium  compound.  Carbohydrate Research138, 357-368.

 Abstract publications and communications

Adikaram C.P., Perera J , Wijesundera S. Mutation in the non-Rifampicin Resistant- Determining Region (RRDR) of the rpoB gene contributes significantly to rifampicin resistance of Mycobacterium tuberculosis strains: the profile from Sri Lanka. ICAAC 2011, Chicago, USA.

Adikaram C.P., Perera J., Wijesundera S. and Weersinghe B.(2010) Prevalence of Tuberculosis  and Rifampicin resistance in Colombo Prisons. The Ceylon Medical Journal, 55, 48.

 Adikaram C.P., Perera J., Wijesundera S. Comparison of Mycobacterium Growth Indicator Tube (MGIT) method and Nitrate Reductase Assay (NRA) with agar proportion method (APM) for detection of Rifampicin resistance. Proceedings of the Sri Lanka Association for the Advancement of Science 64th Annual sessions Abs p 117/A.

Waranakula Kusum De Abrew, K, Wijesundera , S, Wijayadharmadasa, S, Corea , E, and Peiris, S. (2011).  The outcome of use of an interactive distance learning module by students before entry to the medical faculty. 6th congress of the Asian Medical Education Association 23rdto 26th March 2011

Wijesundera S. De Abrew K. Corea E, Peiris S. (2010). Medical Education may begin even before entry to the Midical Faculty. 7th Asia  Pacific Medical Education Conference (APMEC), 4th to 8th February 2010.                 

Wimalasena, G.R.M., Wijesundera, W.S.S. and Vivehananthan, K. (2009). ‘Optimization of Repetative Element Based PCR to Identify Polymorphism in Magnaporthe grisea’. Proceedings of 9th Agricultural Research Symposium, 208-284.

 Dassanayake, C.M., Wijesundera, W.S.S. and Vivehananthan, K. (2009). ‘Primer Designing and PCR amplification of Glutelin Complete Promoter Region in Sri Lankan Rice Variety (Japonica Group). Proceedings of 9th Agricultural Research Symposium, 299-303

 Vaihunthan S. Wijesundera W.S.S., Jayasena S.M.T. and Sirimanne S.R (2008). Construction of rice endosperm specific    expression cassette containing Glu B-1 promoter-GFP reporter-Nos terminator by overlap extension PCR. Proceedings of the Sri Lanka Association for the Advancement of Science 64th Annual sessions Abs p 413/D.

 Jayasena S.H. Gamage S. Wijesundera W.S.S and Perera J. (2006). Identification of Mycobacterium tuberculosis in PCR products of clinical samples by using an oligonucleotide based ELISA detection method.  Proceedings of the 26th annual Sessions of the Institute of Biology, Sri Lanka. Abs.27 p55.

 Wijesundera W.S.S. and de Abrew  K. (2005).  Facilitating Learning Improvement in Students of the Colombo Medical Faculty through Interventional Mechanisms adopted by the Medical Faculty Staff. Staff Development -SLAIHEE conference, University of Colombo.

Wijesundera W.S.S., De Abrew K and Gunatunge W. (2003).  Designing and implementing learning support to address student diversity needs.  The Scholarship of Academic and staff Development: Research Evaluation and Changing Practice. Joint conference organized by the Staff and Educational Development Association and the Society for research into Higher Education.  Bristol 9th -11th April. Abs. p 45.

Wijesundera W.S.S., Dayananda  A.G.L. K. and Karunanayake E. H. (2002).  Preliminary studies on the characterization of a Sri Lankan isolate of Papaya Ringspot Virus (PRSV) genome. Proceedings of the Sri Lanka Association for the Advancement of Science 58th Annual sessions Abs p 18.

Banduseela, V.C., Wijesundera W.S.S. and Karunanayake,E.H. (2001). “Screening of macrodeletions of Duchenne Muscular Dystropy (DMD) patients in Sri Lankan population by a multiplex PCR system”. Proceedings of the Sri Lanka Association for the Advancement of Science, 57th Annual Sessions Abs. A-011, p 11.

Abeywardana, W.D.A.B., Wijesundera, W.S.S., Kodikara, C.P., Abeygunewardena, S.I. and Karunanayake, E.H. (2000). "Detection of Salmonellain raw beef: a ploymerase chain reaction (PCR) assay" Proceedings of the Sri Lanka Association for the Advancement of Science, 56th Annual Sessions Abs. D-220, p 158.

Pathirana, B.N., Wijesundera, W.S.S., Karunanayake, E.H., Jayasena, S.M.T. and Dayananda, A.G.M.L.K. (2000).  "Characterization of a Heat Shock Protein 70 gene of Wuchereria bancrofti" Proceedings of the Sri Lanka Association for the Advancement of Science, 56th Annual Sessions Abs. A-228, p 26.

Jayasinghe, D.R., Wijesundera, W.S.S., Dayananda, A.G.M.L.K., Karunanayake, E.H. and Rajapakse,P.V.J.  (2000). "Comparative analysis of 5S ribosomal RNA intergenic region of three common species of Setaria" Proceedings of the Sri Lanka Association for the Advancement of Science, 56th Annual Sessions Abs. A-252, p 46.

Hettiaratchi, U.P., Karunanayake, E. H., Wijesundera, W.S.S.. Angunawela, P. and Tennekoon, K.H (2000). "Preliminary study on BRCA1 mutation analysis of Sri Lankan breast cancer patients" Proceedings of the Sri Lanka Association for the Advancement of Science, 56th Annual Sessions Abs. A-208, p8.

Wickramasinghe, M.M.,  Karunanayake, E. H., Wijesundera, W.S.S., Tennekoon, K.H. and Angunawela, P. (2000).  "A preliminary study on TP53 tumour suppressor gene mutations in breast cancer patients of Sri Lanka" Proceedings of the Sri Lanka Association for the Advancement of Science, 56th Annual Sessions Abs. A-207, p7.

Gunawardena Y.I.N.S, Wijesundera W.S.S, Siridewa K, Karunanayake E.H and Chandrasekharan N. V. (1997).  Development of a non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti.  Proceedings of the Sri Lanka Association for the Advancement of Science, 53rd Annual Sessions Abs. A-10, p10.

Wijesundera, W.S.S., Chandrasekharan, N.V. and  Karunanayake. E.H., [1993].  Complete sequence of a highly repetitive DNA fragment in Setaria digitata. Proceedings of the Sri Lanka Association for the Advancement of Science 49th Annual sessions Abs p 35.

Wijesundera, W.S.S., Mathew, C.P.D.W. and  Karunanayake. E.H., [1990]. Kinetic studies of Lactate dehydrogenase from Setaria digitats.  Proceedings of the Sri Lanka Association for the Advancement of Science 46th Annual sessions Abs p 3.

Wijesundera, W.S.S., Mathew, C.P.D.W., Karunanayake. E.H. and Dissanayake, S., [1989].  Purification of Lactate dehydrogenase from cattle filarial parasite, Setaria digitata. Proceedings of the Sri Lanka Association for the Advancement of Science 45th Annual sessions Abs p 12.

Awards and Distinctions

George Fleming Prize in 1997, for the most meritorious paper published in The Veterinary Journal, 1996 titled “Development of a diagnostic DNA probe to detect Setaria digitata: The causative parasite of cerebrospinal nematodiasis in goats, sheep and horses”

Merit Award, General Research Committee. Sri Lanka Association for the Advancement of Science (1999). For the research work carried out for the period of1986 to 1998, titled “Development of Diagnostic DNA Probes and Polymerase Chain Reaction based assays for Filarial Parasites” by N.V. Chandrasekharan, E.H. Karunanayake, K. Siridewa, W.S.S. Wijesundera (in alphabetical order)

President’s Research Award 2001. In appreciation for the authorship in Veterinary Parasitology, 81: 225-233, 1999, titled “A sensitive polymerase chain reaction based assay for the detection of Setaria digitata: The causative organism of cerebrospinal nematodiasis in goats, sheep and horses”.

Vice-Chancellor’s Award in Recognition of Excellence in Research, Years 1999,2000/2001. April 2002

 Presidents research award 2003 in appreciation for the authorship in The Veterinary Journal, 165, 136-142, 2003 titled “Differentiation of Setaria digitata and Setaria labiatopapillosa using molecular markers”.

Adikaram C.P , Perera J , Wijesundera S , Senarathne V .(2010) Mutations in the rpoB gene of rifampicin resistant Mycobacterium tuberculosis isolates in Sri Lanka, 2nd annual sessions and symposium, Association of the Pulmonologist (Awarded 1st place form oral presentations).

 

Professional achievements and activities

 Successfully completed the course of study leading to the award of the Accreditation of Senior Teachers in Higher Education of the Staff Development Center, University of Colombo, June 2003. 

 Teaching

Undergraduates

  •   Molecular Biology and Gene technology
  •  Biochemistry   
  •  Facilitate Small group discussions in Community Stream and Behavioural stream of the Medical curriculum

 Postgraduate

  •   Molecular Biology and Gene technology

 Other activities

 Member of the Sri Lanka Association for the advancement of Science.

 Member of the Institute of Chemistry, Sri Lanka, C.Chem., F.I. Chem.

 Member of the institute of Biology, M.I. Biol.

 Professional Commitments

Chairperson, Specialty Board in Molecular Medicine, Postgraduate Institute of Medicine University of Colombo 2007 to date.

 Member of the Board of Study, Multi Disciplinary Study Courses, Postgraduate Institute of Medicine University of Colombo 2007 to       date.

 Member of the Interim Committee for Curriculum Development in Molecular Medicine, Postgraduate Institute of Medicine from 2004-2007.

Course Coordinator, Postgraduate Diploma/M.Sc. Postgraduate Institute of Medicine University of Colombo, 2009 – to date.

Course Coordinator, M.Sc. in Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, Faculty of Medicine, 2006 -2010.

Person in Charge for Improving Trilingual capabilities of Medical Undergraduate and staff under the IRQUE (Improving Relevance and Quality of Undergraduate Education) project, 2004 -2010.

Chairperson, Staff Group for Student Assistance 2002 –2009

Senior Treasure, Faculty of Medicine Buddhist Association, 2005/2006.

Senior Treasure, Medical Faculty Students’ Welfare Society 2004.

Convenor, Nutrition Module, 1998- 2002.

 

Employment

  • Temp. Assistant Lecturer, Oct. 1980-June 1981 Department of Chemistry, University of Colombo.
  • Assistant Analyst, July-1981-Oct. 1981. Department of Government Analyst, Colombo. 
  •  Research Assistant, Feb. 1982- Sept. 1984. Dept. of Organic Chemistry, University of Bristol, England.
  • Assistant Lecturer, April 1985-April 1989. Department of Chemistry, University of Colombo.
  •  Lecturer, April 1989- December 1997. Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Colombo.
  •  Visiting Research Specialist, September 1991- August 1992. Department of Chemistry, University of Illinois at Chicago, U.S.A.
  • Senior   Lecturer Grade II December 1997- May 2004. Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Colombo.
  • Senior   Lecturer Grade I, May 2004- to date. Department of Biochemistry & Molecular Biology, Faculty of Medicine, University of Colombo.

 Supervised projects

  1.  C.K. bandara – 2010. Cloning and characterization of bromelain gene from pineapple.
  2. N. Hedellage -2010. Study of factor V Hong Kong and factor V Cambridge mutations in a group of Sri Lankans presenting with deep vein thrombosis.
  3. M. A. Dimutu Chamara- 2010. Isolation and cloning of the cry IVD gene of Bacillus thruringienisis subsp israelensis as a preliminary step in developing a mosquitocidal garden house plant.
  4. D.S.D. Rajapakse-2010. PCR assay to screen for fragile x syndrome among children having learning difficulties.
  5.  K. Manikkasinghe-2010. Preliminary studies on the cloning of cellulose genes from Aspergillus niger.
  6.  P. Kenthesswaran -2008. M.Sc. thesis titled ‘Identification of nematode specific fatty acid and retinol binding protein encoding gene far- 1 from filarial parasitic nematode Setaria digitata’.
  7.  U. Srimydoran-2008. M.Sc. thesis titled ‘Preliminary study of HFE gene mutations in patients with clinical evidences suggestive of iron overload’.
  8. S. Vaihunthan-2008. M.Sc. thesis titled ‘Construction of rice endosperm specific expression cassette containing Glu B-1 promoter-GFP reporter-Nos terminator by overlap extension PCR’.
  9. S.H. Jayasena -2005. M.Sc. thesis titled ‘Development of an oligonucleotide based ELISA detection method to identify Mycobacterium tuberculosis in PCR products of clinical samples’.
  10. Yamuna K.W. Pathirana-2005. M.Sc. thesis titiled ‘Polymerase Chain Reaction (PCR) amplification and cloning of Putative Abundant Larval Transcript (alt) Genes of Setaria digitata’.
  11. D. R. Jayasinghe- 2000. M.Sc. thesis titled ‘Differentiation of Setaria digitata and Setaria labiatopapillosa using the 5S ribosomal RNA intergenic  region and Random amplified polymorphic DNA (RAPD) analysis’
  12. W. D. A. B. Abeywardana – 2000, M.Sc. thesis titled ‘A polymerase chain reaction based assay for the detection of Salmonella in raw ground beef’.
  13. Y.I.N.S. Gunawardena – 1997. M.Sc. thesis titled ‘A simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti’.

 

Contact information

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