Dr. Kithmini Siridewa (Senior Lecturer Grade I)

Head of Department

 

CONTACT INFORMATION

Office - 2697947 , Mobile - 0714488471

Email - kithmini@bmb.cmb.ac.lk


  Academic Record

 B.Sc.,1984, in Biological sciences (University of Colombo)

M.Sc., 1989 in Bochemistry, Molecular biology & Gene technology (University of Colombo)

Ph.D. 1999 in Molecular Biology & Gene Technology (University of Colombo)

 

Research Interests  

  • Molecular Parasitology of Filarial nematode Wuchereria bancrofti
  • Molecular cloning and characterisation of parasite specific genes, coding for host - protective antigensfrom the human filraial parasite Wuchereria bancrofti
  • Biological effects of 25-hydroxyvitamin D3 (calcidiol) administration on bone mineral metabolism.
  • Purification and characterization of glycoproteins for vaccines.


Publications

Book chapters

·    Unit Author (2009) to the Course book on ‘Cell Biology and Basic Biochemistry’- MLU 1246. Open University of Sri Lanka.

 

Unit 8 : Fat and Lipid metabolism 3 chapter

i.Basic Biochemistry of lipids with their functions.

ii.Lipoproteins & their biological role and disorders in lipoprotein metabolism.

iii.Measurement of lipids and apolipoprotein with clinical significance.

 

Unit 9: Nucleic acid & their metabolism 3 chapters

i.  Chemistry and structure of nucleic acids

ii. Purine and pirimidine metabolism.

iii Disorders of purine and pirimidine metabolism.

 

List of Publications

Gunawardena, Y.I.N.S. Wijesundera, W.S.S., Karunanayake, E.H., Chandrasekharan, N.V., Jayasekera, N. & Siridewa, K. (1999) A simple and rapid non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti Southeast Asian Journal of Tropical Medicine & Public Health 30, 350-355.

 

Siridewa, K., Karunanayake, E.H. & Chandrasekharan, N.V. (1996). Polymerase Chain Reaction-based technique for the detection of Wuchereria bancrofti in human blood samples, hydrocele fluid, and mosquito vectors. American Journal of Tropical Medicine & Hygiene 54, 72-76.

 

 Siridewa, K., Karunanayake, E.H., Chandrasekharan, N.V., Franczen, L. & Pettersson, U.(1994). Cloning and characterization of a repetitive DNA sequence specific for Wuchereria bancrofti American Journal of Tropical Medicine & Hygiene 51, 495- 500.

 

Gilljam, G., Siridewa, K. & Hammar, L. (1994). Purification of simian immunodeficiency virus, SIV,and of its external envelope glycoprotein, gp 148. Journal of Chromatography A, 675, 89-100.

 

Siridewa, K., Froman, G., Hammar, L. & Mardh, P. (1993). Characterization of glycoproteins from Chlamydia trachomatis using lectins. APMIS, 101, 851–857.

 

 Abstract publications and communications

Siridewa Kithmini, Froman Gunnar, Hammar Lena and Mardh Per-Anders. Characterization of glycoproteins from Chlamydia trachomatis using lectins. Proceedings (2) of the European Society for Chlamydia Research 1992; Stockholm, Sweden.

Karunanayake EH, Siridewa K and Chandrasekharan NV. A species specific DNA probe for Wuchereria bancrofti. An international conference on Biotechnology and Environmental Science: Molecular Approaches 1990 Aug. 21-24; Chulaborn Research.

 Pelawatte TC, Siridewa K and Karunanayake EH. Partial characterization of 6.4 kb repeat from Wuchereria bancrofti genome. Proceedings of the Sri Lanka Association for the Advancement of Science 1998; 54rd Annual sessions, Sri Lanka.

Gunawardena YINS, Wijesundera WSS, Siridewa K, Karunanayake EH and Chandrasekharan NV. Development of a non-radioactive oligonucleotide based hybridization assay for the detection of Wuchereria bancrofti. Proceedings of the Sri Lanka Association for the Advancement of Science 1997; 53rd Annual sessions, Sri Lanka.

 Kanagaratnam BL, Hewage UCL, Siridewa K, Karunanayake EH and Weerasuriya K. Hydrocele: The filarial connection Sri Lanka Medical Association 1991; 104 th Anniversary Academic sessions, Sri Lanka.

 

 Siridewa K, Karunanayake EH, Premaratne U, Abeyewickreme W and Ismail MM. Detection of Wuchereria bancrofti microfilariae in human blood samples using a species specific DNA probe. Proceedings of the Sri Lanka Association for the Advancement of Science 1990;46th Annual sessions, Sri Lanka.

 

Siridewa K, Karunanayake EH, Abeyewickreme W, Premaratne U, Ismail MM, and Jayasekera N. Detection of L3 larvae of Wuchereria bancrofti using species specific DNA probe. Proceedings of the Sri Lanka Association for the Advancement of Science 1990;46th Annual sessions, Sri Lanka.

 

Siridewa K, Chandrasekharan NV, Karunanayake EH and Dissanayake S. A moderately repeated DNA sequence from Wuchereria bancrofti. Proceedings of the Sri Lanka Association for the Advancement of Science 1989;45th Annual sessions, Sri Lanka.

 

Karunanayake EH, Chandrasekharan NV, Dissanayake S, Siridewa K, Franzen L and Pettersson U. Cloning and partial characterization of repetitive sequences from Wuchereria bancrofti. Proceedings of the Sri Lanka Association for the Advancement of Science 1988; 44th Annual sessions, Sri Lanka.

 

Awards and Distinctions

Merit Award, General Research Committee. Sri Lanka Association for the Advancement of Science (1999). For the research work carried out for the period of1986 to 1998, titled “Development of Diagnostic DNA Probes and Polymerase Chain Reaction based assays for Filarial Parasites” by N.V. Chandrasekharan, E.H. Karunanayake, K. Siridewa, W.S.S. Wijesundera (in alphabetical order)

 

Professional achievements and activities

    • Certificate in Teaching in Higher Education (CTHE), 2001. University of Colombo.
 
  • International Programmed in Chemical Sciences (IPICS) fellowship to gain experience in Molecular biology & Biochemistry at the Biomedical center, University of Uppsala,Sweden. 
 
Teaching

Undergraduates

  • Molecular Biology and Gene technology
  • Biochemistry
  • Facilitate Small group discussions in Community Stream and Behavioural stream of the Medical curriculum


Postgraduate

  • Molecular Biology and Gene technology


Other activities

Member of the Sri Lanka Association for the advancement of Science.

Member of the institute of Biology, M.I. Biol.

 

Professional Commitments

Convenor Trauma Module Faculty of Medicine, Colombo, 2000-2003.

Deputy Coordinator of the IRQUE project Faculty of Medicine, Colombo, 2007

 

Supervised projects

Completed MSc Students:

Pelawatte, T.C – 1997. “Characterization of 6.4 kb repeat from Wucheria bancrofti genome”.

Disanayake, A.S – 2000. Characterization of 6.4 kb repeat DNA fragment from Wuchereria bancrofti.

 

 Contact information

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Tel.0094112697947

Fax 0094112697485